{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE338nnn/GSE338028/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE338028"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Lipid-based mRNA chimeric antigen receptor T cells for enhanced in vitro antitumor activity","description":"Chimeric antigen receptor (CAR) T cell therapy provides substantial clinical benefits in hematologic malignancies. However, cell engineering using conventional viral vectors is costly, labor-intensive, and prone to safety risks, while electroporation-induced cellular damage limits manufacturing scalability. This study reports a safe, high-efficiency, nonviral platform for generating mRNA-engineered CAR-T cells. Herein, eight ionizable lipids are synthesized and formulated into lipid nanoparticles. Systematic screening and structural optimization identified Lipid 6 as the lead candidate. Transfection efficiency is validated by delivering eGFP mRNA into DC2.4, peripheral blood mononuclear, dendritic, and natural killer cells. Murine models confirm robust in vivo expression and a favorable safety profile. Lipid 6–mediated delivery of MUC1 mRNA generates MUC1–CAR-T cells characterized by high surface receptor expression and potent cytotoxicity against MUC1-positive tumor cells. These findings establish Lipid 6 as a feasible strategy for primary human T-cell engineering, facilitating functional CAR expression with minimal toxicity.","dates":{"publication":"2026/08/12"},"accession":"GSE338028","cross_references":{"GSM":["GSM9865020","GSM9865019","GSM9865018","GSM9865017","GSM9865022","GSM9865021"],"GPL":["24676"],"GSE":["338028"],"taxon":["Homo sapiens"]}}