GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE338nnn/GSE338200/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE338200GEOGSEfalseCannabidiol reverses microglial exhaustion in aged Gulf War Illness miceGulf War Illness (GWI) affects up to one-third of 1990–1991 Gulf War veterans and is associated with chronic low-grade neuroinflammation, yet its cellular substrates remain unclear and targeted pharmacological therapies are still limited. By using bulk RNA-seq analyses of FACS-purified microglia in an established GWI mouse model, we uncovered a multi-lineage primed neuroimmune signature in aged GWI mice. Systemic lipopolysaccharide (LPS) elicited an amplified pro-inflammatory response in the brains of GWI mice compared to age-matched controls, yet paradoxically, microglia were transcriptionally hyporesponsive to LPS, with inverted antigen presentation module score indicative of a microglial exhaustion phenotype. Chronic cannabidiol (CBD) attenuated neuroimmune priming at baseline, reshaping microglial translational and energetic states. Under immune-stimulated conditions, CBD partially restored GWI-associated deficits in microglial antigen presentation and oxidative phosphorylation, defining a therapeutic axis amenable to pharmacological intervention.2026/07/14GSE338200GSM9869178GSM9869167GSM9869166GSM9869177GSM9869176GSM9869187GSM9869186GSM9869175GSM9869185GSM9869174GSM9869173GSM9869184GSM9869183GSM9869172GSM9869171GSM9869182GSM9869181GSM9869170GSM9869180GSM9869169GSM9869168GSM986917924247338200Mus musculus