{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE338nnn/GSE338237/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Caenorhabditis elegans"],"gds_type":[" Other","Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE338237"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"The Z-granule scaffold LOTR-1 bridges piRNA recognition to small-RNA amplification in C. elegans","description":"LOTR-1 is a Caenorhabditis elegans member of the conserved LOTUS- and Tudor-domain (TDRD5/7-like) family that resides in a germ granule subcompartment (Z granule). To dissect how LOTR-1 and its individual domains shape gene expression and translation, we performed paired total-lysate and polysome-associated mRNA sequencing across three domain-specific alleles: a full in-frame deletion (delta-LOTR-1), a LOTUS-domain deletion (delta-LOTUS), and a Tudor-domain deletion (delta-Tudor), each compared to a wildtype control. Differential expression analysis reveals widespread transcriptomic remodeling in all three alleles, strongest in the LOTUS-domain deletion, that is extensively offset by genome-wide translational buffering, including a coordinated downregulation of ribosomal protein mRNAs. The MiDAC scaffold saeg-2 is robustly derepressed across all alleles. These paired lysate and polysome datasets provide a read-out of both transcript abundance and translational engagement in lotr-1 domain mutants.","dates":{"publication":"2026/07/10"},"accession":"GSE338237","cross_references":{"GSM":["GSM9869772","GSM9869750","GSM9869770","GSM9869759","GSM9869758","GSM9869757","GSM9869777","GSM9869755","GSM9869776","GSM9869754","GSM9869775","GSM9869753","GSM9869774","GSM9869752","GSM9869751","GSM9869773","GSM9869761","GSM9869760","GSM9869749","GSM9869748","GSM9869769","GSM9869747","GSM9869768","GSM9869746","GSM9869745","GSM9869767","GSM9869766","GSM9869744","GSM9869765","GSM9869764","GSM9869763","GSM9869762"],"GPL":["34620"],"GSE":["338237"],"taxon":["Caenorhabditis elegans"]}}