GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE338nnn/GSE338237/primaryOK200TranscriptomicsCaenorhabditis elegans OtherExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE338237GEOGSEfalseThe Z-granule scaffold LOTR-1 bridges piRNA recognition to small-RNA amplification in C. elegansLOTR-1 is a Caenorhabditis elegans member of the conserved LOTUS- and Tudor-domain (TDRD5/7-like) family that resides in a germ granule subcompartment (Z granule). To dissect how LOTR-1 and its individual domains shape gene expression and translation, we performed paired total-lysate and polysome-associated mRNA sequencing across three domain-specific alleles: a full in-frame deletion (delta-LOTR-1), a LOTUS-domain deletion (delta-LOTUS), and a Tudor-domain deletion (delta-Tudor), each compared to a wildtype control. Differential expression analysis reveals widespread transcriptomic remodeling in all three alleles, strongest in the LOTUS-domain deletion, that is extensively offset by genome-wide translational buffering, including a coordinated downregulation of ribosomal protein mRNAs. The MiDAC scaffold saeg-2 is robustly derepressed across all alleles. These paired lysate and polysome datasets provide a read-out of both transcript abundance and translational engagement in lotr-1 domain mutants.2026/07/10GSE338237GSM9869772GSM9869750GSM9869770GSM9869759GSM9869758GSM9869757GSM9869777GSM9869755GSM9869776GSM9869754GSM9869775GSM9869753GSM9869774GSM9869752GSM9869751GSM9869773GSM9869761GSM9869760GSM9869749GSM9869748GSM9869769GSM9869747GSM9869768GSM9869746GSM9869745GSM9869767GSM9869766GSM9869744GSM9869765GSM9869764GSM9869763GSM986976234620338237Caenorhabditis elegans