{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE338nnn/GSE338396/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Methylation profiling"],"species":["Arabidopsis thaliana"],"gds_type":["Methylation profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE338396"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Protocol for low-input whole-genome bisulfite sequencing in plants","description":"DNA methylation profiling of rare plant samples is limited by the scarcity of input DNA. This protocol uses Arabidopsis leaf to describe tissue cryo-milling, proteinase K lysis, direct bisulfite conversion, post-bisulfite adapter tagging library construction with random priming and adapter ligation, and MethylStar-based alignment and methylation calling. DNA inputs as low as 50 pg generate methylomes comparable to conventional 50-ng WGBS.","dates":{"publication":"2026/07/13"},"accession":"GSE338396","cross_references":{"GSM":["GSM9872896","GSM9872897","GSM9872895","GSM9872900","GSM9872911","GSM9872912","GSM9872901","GSM9872898","GSM9872899","GSM9872910","GSM9872904","GSM9872905","GSM9872902","GSM9872903","GSM9872908","GSM9872909","GSM9872906","GSM9872907"],"GPL":["34544"],"GSE":["338396"],"taxon":["Arabidopsis thaliana"]}}