GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE338nnn/GSE338396/primaryOK200Methylation profilingArabidopsis thalianaMethylation profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE338396GEOGSEfalseProtocol for low-input whole-genome bisulfite sequencing in plantsDNA methylation profiling of rare plant samples is limited by the scarcity of input DNA. This protocol uses Arabidopsis leaf to describe tissue cryo-milling, proteinase K lysis, direct bisulfite conversion, post-bisulfite adapter tagging library construction with random priming and adapter ligation, and MethylStar-based alignment and methylation calling. DNA inputs as low as 50 pg generate methylomes comparable to conventional 50-ng WGBS.2026/07/13GSE338396GSM9872896GSM9872897GSM9872895GSM9872900GSM9872911GSM9872912GSM9872901GSM9872898GSM9872899GSM9872910GSM9872904GSM9872905GSM9872902GSM9872903GSM9872908GSM9872909GSM9872906GSM987290734544338396Arabidopsis thaliana