GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE338nnn/GSE338608/primaryOK200TranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE338608GEOGSEfalseSingle-cell RNA-seq of out-of-thaw bone marrow-derived mesenchymal stromal cells (BM-MSCs)Human mesenchymal stromal cells (MSCs) used in cell therapy are frequently administered directly out of thaw, yet the single-cell transcriptomic landscape of out-of-thaw MSC products is poorly defined. Here, cryopreserved bone marrow-derived MSCs from six healthy donors (seven lots) were characterized immediately after thawing using droplet-based single-cell RNA-sequencing on the Illumina-Bio-Rad ddSEQ platform. After SureCell-based UMI counting and knee filtering, profiles were analyzed with SC3 and Seurat. Donor-level clusters were identified that differ in immune-signaling, cell-surface, cell-cycle and metabolic gene programs, with low within-sample heterogeneity; cell-cycle status emerged as a major axis of inter-donor variation. Only the bone marrow samples are included in this submission.2026/07/14GSE338608GSM9878143GSM9878148GSM9878149GSM9878144GSM9878145GSM9878146GSM987814718573338608Homo sapiens[34736534]