GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE339nnn/GSE339083/primaryOK200GenomicsHomo sapiensNon-coding RNA profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE339083GEOGSEfalseEffect of TENT2 knockdown on miRNA profiling in human lung endothelial cellsMiRNAs are generated through a finely tuned multi-step process that includes precise cropping and cleavage of their precursors. The mature miRNA is then loaded onto an argonaute (Ago) protein to form an RNA-induced silencing complex (RISC) and target messenger RNAs. Most of the mechanisms of miRNA regulation target one of the steps in the biogenesis. However, through analysis of miR-1 precursors, we demonstrate that VEGF stimulation induces degradation (decay) of mature miR-1-3p without affecting its precursors. This analysis also identified a specific miRNA 3’-adenylation pathway that targets the guide strand after RISC loading. VEGF induced the expression of terminal nucleotidyl transferase 2 (TENT2, also known as PAPD4 or GLD2), one of the main enzymes that mediate 3’ miRNA adenylation, and knockdown of TENT2 inhibited miR-1 decay. We examined the biological significance of TENT2 in VEGF-mediated EC proliferation, its pathogenic role in murine NSCLC models, and its expression and clinical significance in human NSCLC.2026/07/22GSE339083GSM9888182GSM9888185GSM9888186GSM9888183GSM9888184GSM988818734281339083Homo sapiens