GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE339nnn/GSE339100/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE339100GEOGSEfalsesc-RNAseq of glomerular cells in a mouse model of malignant hypertensionHypertensive emergency (HTEM) is defined by abrupt blood pressure elevation with acute multi‑organ damage, yet the mechanisms predisposing only a subset of hypertensive individuals to HTEM remain unclear. Progress has been limited by the lack of a mouse model that faithfully replicates human disease. We aimed to identify determinants of susceptibility to hypertensive microvascular injury and characterize a murine model of HTEM. Males 129S2/SvPasCrl (129Sv) mice were exposed to severe hypertension via angiotensin II infusion combined with a high‑salt diet wth or withour supplementation with rhPlGF2. We assessed glomerular transcriptional profiles using single‑cell RNA sequencing. Single‑cell transcriptomics revealed profound repression of angiogenic, metabolic, and stress-response pathways in glomerular endothelial cells, a repression partially restored by rhPlGF-2.2026/09/01GSE339100GSM9888482GSM9888483GSM9888480GSM9888481GSM9888479GSM9888477GSM988847830172339100Mus musculus