GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE339nnn/GSE339192/primaryOK200TranscriptomicsSaccharomyces cerevisiaeExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE339192GEOGSEfalseChaperone condensates buffer the heat shock response against pleiotropic inputs [RNA-seq]RNA-seq of Saccharomyces cerevisiae profiling the heat shock response (HSR) across chemically distinct stresses and upon acute nuclear depletion of the co-chaperone Sis1. Wild-type cells were profiled unstressed and under heat shock, sodium arsenite, azetidine-2-carboxylic acid (AZC), diazaborine (DZA), and hyperosmotic NaCl, each with and without cycloheximide (CHX) to test translation-dependence. In parallel, a rapamycin-resistant Sis1 anchor-away strain was profiled with and without rapamycin across the same stresses. Only heat shock and, weakly, arsenite induce HSR target genes; nuclear Sis1 buffers HSR output under sub-saturating stress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 cerevisiae[42619928]