{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE339nnn/GSE339279/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"species":["Homo sapiens"],"gds_type":["Non-coding RNA profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE339279"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Characterizing circular and linear non-coding RNA in human aging brains and their association with dementia","description":"Total RNA, depleted for ribosomal RNA, is commonly used for RNA sequencing to capture both polyadenylated and non-polyadenylated transcripts, while the poly(A) tail targeting protocols can either enrich (Poly(A)-selection) or deplete (Poly(A)-depletion) the polyadenylated fraction. To ascertain the best library strategy for capturing long non-coding RNAs (lncRNAs, >200nt) and circular RNAs (circRNAs) in aged brains, we applied three protocols (total ribo-depleted, poly(A)-selected, and poly(A)-depleted) to 128 human hippocampus samples. Our results show that both ribo-depleted and poly(A)-depleted perform well for lncRNA and circRNA profiling, with poly(A)-depleted protocol generally having a slightly higher number of non-coding RNAs detected. Additionally, we compared poly(A)-selected and -depleted data for predicting the poly(A) status of hippocampus transcripts. The proportion of non-polyadenylated transcripts was higher than previously found. This study demonstrates how the choice of library construction impacts the outcomes, helping to make informed methodological choices when studying brain non-coding RNAs. In addition, we for the first time examined whether the poly(A) status of protein-coding mRNAs is associated with AD and AD-related dementias.","dates":{"publication":"2026/07/20"},"accession":"GSE339279","cross_references":{"GSM":["GSM9892733","GSM9892612","GSM9892613","GSM9892734","GSM9892610","GSM9892731","GSM9892611","GSM9892732","GSM9892730","GSM9892609","GSM9892607","GSM9892728","GSM9892608","GSM9892729","GSM9892605","GSM9892726","GSM9892727","GSM9892606","GSM9892724","GSM9892603","GSM9892604","GSM9892725","GSM9892744","GSM9892502","GSM9892623","GSM9892624","GSM9892745","GSM9892503","GSM9892621","GSM9892742","GSM9892500","GSM9892501","GSM9892622","GSM9892743","GSM9892740","GSM9892620","GSM9892741","GSM9892618","GSM9892739","GSM9892619","GSM9892616","GSM9892737","GSM9892617","GSM9892738","GSM9892614","GSM9892735","GSM9892736","GSM9892615","GSM9892711","GSM9892712","GSM9892710","GSM9892708","GSM9892709","GSM9892706","GSM9892707","GSM9892704","GSM9892705","GSM9892702","GSM9892703","GSM9892601","GSM9892722","GSM9892602","GSM9892723","GSM9892720","GSM9892600","GSM9892721","GSM9892719","GSM9892717","GSM9892718","GSM9892715","GSM9892716","GSM9892713","GSM9892714","GSM9892535","GSM9892656","GSM9892414","GSM9892415","GSM9892536","GSM9892657","GSM9892412","GSM9892533","GSM9892654","GSM9892413","GSM9892534","GSM9892655","GSM9892410","GSM9892531","GSM9892652","GSM9892653","GSM9892411","GSM9892532","GSM9892650","GSM9892530","GSM9892651","GSM9892409","GSM9892407","GSM9892528","GSM9892649","GSM9892408","GSM9892529","GSM9892647","GSM9892405","GSM9892526","GSM9892527","GSM9892648","GSM9892406","GSM9892660","GSM9892667","GSM9892425","GSM9892547","GSM9892668","GSM9892426","GSM9892665","GSM9892423","GSM9892424","GSM9892545","GSM9892666","GSM9892421","GSM9892663","GSM9892664","GSM9892422","GSM9892543","GSM9892661","GSM9892540","GSM9892541","GSM9892662","GSM9892420","GSM9892418","GSM9892539","GSM9892419","GSM9892416","GSM9892537","GSM9892658","GSM9892538","GSM9892659","GSM9892417","GSM9892513","GSM9892634","GSM9892755","GSM9892514","GSM9892635","GSM9892753","GSM9892511","GSM9892632","GSM9892633","GSM9892754","GSM9892512","GSM9892630","GSM9892751","GSM9892510","GSM9892631","GSM9892752","GSM9892750","GSM9892508","GSM9892629","GSM9892509","GSM9892627","GSM9892748","GSM9892506","GSM9892507","GSM9892628","GSM9892749","GSM9892504","GSM9892625","GSM9892746","GSM9892747","GSM9892505","GSM9892626","GSM9892524","GSM9892645","GSM9892403","GSM9892404","GSM9892525","GSM9892646","GSM9892401","GSM9892522","GSM9892643","GSM9892644","GSM9892402","GSM9892523","GSM9892641","GSM9892520","GSM9892521","GSM9892642","GSM9892400","GSM9892640","GSM9892519","GSM9892517","GSM9892638","GSM9892518","GSM9892639","GSM9892515","GSM9892636","GSM9892516","GSM9892637","GSM9892700","GSM9892701","GSM9892395","GSM9892396","GSM9892393","GSM9892394","GSM9892391","GSM9892392","GSM9892390","GSM9892399","GSM9892397","GSM9892398","GSM9892450","GSM9892692","GSM9892693","GSM9892330","GSM9892451","GSM9892690","GSM9892691","GSM9892458","GSM9892337","GSM9892338","GSM9892459","GSM9892335","GSM9892456","GSM9892698","GSM9892336","GSM9892699","GSM9892457","GSM9892333","GSM9892696","GSM9892454","GSM9892455","GSM9892697","GSM9892334","GSM9892452","GSM9892573","GSM9892331","GSM9892694","GSM9892332","GSM9892453","GSM9892695","GSM9892328","GSM9892449","GSM9892329","GSM9892461","GSM9892582","GSM9892340","GSM9892341","GSM9892462","GSM9892583","GSM9892581","GSM9892460","GSM9892348","GSM9892469","GSM9892349","GSM9892467","GSM9892588","GSM9892346","GSM9892347","GSM9892468","GSM9892589","GSM9892344","GSM9892465","GSM9892586","GSM9892587","GSM9892345","GSM9892466","GSM9892584","GSM9892342","GSM9892463","GSM9892464","GSM9892585","GSM9892343","GSM9892339","GSM9892670","GSM9892671","GSM9892315","GSM9892436","GSM9892557","GSM9892678","GSM9892316","GSM9892679","GSM9892437","GSM9892313","GSM9892676","GSM9892434","GSM9892555","GSM9892435","GSM9892677","GSM9892314","GSM9892432","GSM9892553","GSM9892674","GSM9892311","GSM9892312","GSM9892433","GSM9892675","GSM9892430","GSM9892551","GSM9892672","GSM9892673","GSM9892310","GSM9892431","GSM9892308","GSM9892429","GSM9892309","GSM9892306","GSM9892427","GSM9892669","GSM9892307","GSM9892428","GSM9892549","GSM9892681","GSM9892561","GSM9892682","GSM9892440","GSM9892680","GSM9892447","GSM9892326","GSM9892689","GSM9892327","GSM9892448","GSM9892569","GSM9892687","GSM9892324","GSM9892445","GSM9892567","GSM9892688","GSM9892325","GSM9892446","GSM9892685","GSM9892322","GSM9892443","GSM9892444","GSM9892565","GSM9892323","GSM9892686","GSM9892441","GSM9892683","GSM9892320","GSM9892321","GSM9892684","GSM9892442","GSM9892563","GSM9892319","GSM9892438","GSM9892559","GSM9892317","GSM9892318","GSM9892439","GSM9892373","GSM9892494","GSM9892495","GSM9892374","GSM9892371","GSM9892492","GSM9892372","GSM9892493","GSM9892490","GSM9892370","GSM9892491","GSM9892379","GSM9892498","GSM9892377","GSM9892378","GSM9892499","GSM9892375","GSM9892496","GSM9892376","GSM9892497","GSM9892384","GSM9892385","GSM9892382","GSM9892383","GSM9892380","GSM9892381","GSM9892388","GSM9892389","GSM9892386","GSM9892387","GSM9892593"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