GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE339nnn/GSE339279/primary200OKGenomicsHomo sapiensNon-coding RNA profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE339279GEOGSEfalseCharacterizing circular and linear non-coding RNA in human aging brains and their association with dementiaTotal RNA, depleted for ribosomal RNA, is commonly used for RNA sequencing to capture both polyadenylated and non-polyadenylated transcripts, while the poly(A) tail targeting protocols can either enrich (Poly(A)-selection) or deplete (Poly(A)-depletion) the polyadenylated fraction. To ascertain the best library strategy for capturing long non-coding RNAs (lncRNAs, >200nt) and circular RNAs (circRNAs) in aged brains, we applied three protocols (total ribo-depleted, poly(A)-selected, and poly(A)-depleted) to 128 human hippocampus samples. Our results show that both ribo-depleted and poly(A)-depleted perform well for lncRNA and circRNA profiling, with poly(A)-depleted protocol generally having a slightly higher number of non-coding RNAs detected. Additionally, we compared poly(A)-selected and -depleted data for predicting the poly(A) status of hippocampus transcripts. The proportion of non-polyadenylated transcripts was higher than previously found. This study demonstrates how the choice of library construction impacts the outcomes, helping to make informed methodological choices when studying brain non-coding RNAs. In addition, we for the first time examined whether the poly(A) status of protein-coding mRNAs is associated with AD and AD-related dementias.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 sapiens