{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE339nnn/GSE339309/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE339309"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Thymic age and peripheral residence time determine anti-tumor competence of CD8+ T cells","description":"The developmental timing of T cell generation imprints durable functional programs, yet how this shapes anti-tumor immunity remains unclear. Here, we combine genetic fate mapping with functional assays to dissect how thymic age and peripheral residency regulate CD8⁺ T cell behaviour within the same host. We find that, compared with adulthood-derived CD8⁺ T cells, the adolescent-derived counterparts consistently exhibit enhanced tumor infiltration, increased effector cytokine production, and superior proliferative fitness. Transcriptomic and phenotypic profiling identify a CXCR3⁺IL-18Rα⁺ subset preferentially enriched among adolescent-derived T cells that shares core virtual memory-like features and displays elevated cytotoxic potential. Mechanistically, thymic origin timing and time spent in the periphery independently regulate the abundance and activity of this subset, revealing a two-tier control comprising developmental bias and post-thymic remodelling. Functionally, CXCR3⁺IL-18Rα⁺ CD8⁺ T cells mediate potent tumor killing and confer robust therapeutic benefit in adoptive transfer models. Together, these findings establish developmental imprinting as an important determinant of CD8⁺ T cell heterogeneity and identify CXCR3⁺IL-18Rα⁺ CD8⁺ T cells as key effectors for anti-tumor immunity.","dates":{"publication":"2026/09/01"},"accession":"GSE339309","cross_references":{"GSM":["GSM9893329","GSM9893327","GSM9893328","GSM9893325","GSM9893326","GSM9893323","GSM9893324","GSM9893322"],"GPL":["30172"],"GSE":["339309"],"taxon":["Mus musculus"]}}