{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE339nnn/GSE339360/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":[" Mus musculus","Mycobacterium tuberculosis"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE339360"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Dual host-pathogen RNA sequencing of wild-type and EZH2-deficient Hoxb8-derived macrophages during Mycobacterium tuberculosis infection","description":"Polycomb repressive complex 2 (PRC2) and its catalytic subunit EZH2 regulate macrophage permissiveness to intracellular Mycobacterium tuberculosis (Mtb). To define the host and bacterial transcriptional responses associated with EZH2 loss, we performed dual host-pathogen RNA sequencing of parental Cas9-expressing Hoxb8-derived macrophages and CRISPR-generated EZH2-deficient macrophages under uninfected conditions and at 3 days after infection with Mtb Erdman smyc'::mCherry. Host and pathogen transcriptomes were quantified to identify macrophage programs associated with bacterial restriction and bacterial stress-adaptation responses.","dates":{"publication":"2026/07/27"},"accession":"GSE339360","cross_references":{"GSM":["GSM9894167","GSM9894166","GSM9894165","GSM9894164","GSM9894163","GSM9894162","GSM9894161","GSM9894172","GSM9894171","GSM9894170","GSM9894169","GSM9894168"],"GPL":["34461","24247"],"GSE":["339360"],"taxon":[" Mus musculus","Mycobacterium tuberculosis"]}}