{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE339nnn/GSE339493/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE339493"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Interleukin-33 activated group 2 innate lymphoid cells drive type 2 immune dysfunction in endometriosis","description":"Immune dysfunction is a key contributor to endometriosis (EM) pathophysiology, with a type 2-skewed immune response thought to support EM lesion maintenance and establishment. The alarmin interleukin-33 (IL-33) is elevated in EM patient plasma, peritoneal fluid (PF), and lesions compared to controls, and in an EM mouse model IL-33 drives lesion progression, although exact mechanisms remain unclear. Here we validate that IL-33/ST2 signaling contributes to EM pathophysiology, identifying IL-33/ST2 axis dysregulation in EM lesions compared to control endometrial tissue and characterize PF group 2 innate lymphoid cells (ILC2s) with upregulation of ST2+ in severe stage patients. Using ILC2-deficient mice, we demonstrate that ILC2s are critical for the IL-33-driven inflammatory response in EM. IL-33 treatment in EM-induced ILC2-deficient mice resulted in reduced type 2 effector cytokine production and dysregulated immune infiltration, as well as an altered lesion transcriptional signature compared to ILC2-sufficient controls. Notably, reintroducing ILC2s was insufficient to rescue the IL-33-mediated response; however, we identified ILC2 homing to EM lesions. Finally, we identify ST2 neutralization as a promising therapeutic technique to blunt the IL-33/ILC2 type 2 response in EM.","dates":{"publication":"2026/08/03"},"accession":"GSE339493","cross_references":{"GSM":["GSM9896533","GSM9896511","GSM9896512","GSM9896534","GSM9896535","GSM9896513","GSM9896536","GSM9896514","GSM9896515","GSM9896537","GSM9896538","GSM9896516","GSM9896539","GSM9896517","GSM9896518","GSM9896530","GSM9896531","GSM9896532","GSM9896510","GSM9896508","GSM9896509","GSM9896544","GSM9896522","GSM9896523","GSM9896545","GSM9896546","GSM9896524","GSM9896547","GSM9896525","GSM9896526","GSM9896548","GSM9896527","GSM9896528","GSM9896529","GSM9896540","GSM9896541","GSM9896520","GSM9896542","GSM9896543","GSM9896521","GSM9896519"],"GPL":["24247"],"GSE":["339493"],"taxon":["Mus musculus"]}}