{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE341nnn/GSE341225/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Other"],"species":["Homo sapiens"],"gds_type":["Other"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE341225"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Spatial analysis reveals the evolving organization of IDH-mutant glioma","description":"Adult diffuse gliomas comprise malignant cell states interwoven with the non-malignant brain microenvironment. Here, we combine spatial transcriptomics and spatial proteomics of IDH-mutant gliomas to define organizational principles across histological grades. In low-grade tumors, spatial organization is shaped by underlying brain anatomy. We identify a functional white–grey matter junction that restricts cortical invasion and is associated with marked changes in tumor composition and cellular phenotypes. This boundary is preferentially traversed by oligodendrocyte progenitor (OPC)-like malignant cells, suggesting a role in tumor expansion. In contrast, intermediate-grade tumors are largely disorganized, with few recurring interactions between cancer cell states and microenvironmental cell types. In high-grade tumors, hypoxia-associated structure emerges, resembling IDH-wildtype glioblastoma. Together, these findings reveal two independent axes of spatial organization—from anatomy-driven structure in low-grade tumors to hypoxia-driven organization in high-grade tumors—and establish a framework linking tumor grade to recurrent spatial interactions.","dates":{"publication":"2026/07/28"},"accession":"GSE341225","cross_references":{"GSM":["GSM9902430","GSM9902431","GSM9902429","GSM9902425","GSM9902436","GSM9902437","GSM9902426","GSM9902427","GSM9902438","GSM9902428","GSM9902421","GSM9902432","GSM9902433","GSM9902422","GSM9902434","GSM9902423","GSM9902424","GSM9902435"],"GPL":["24676"],"GSE":["341225"],"taxon":["Homo sapiens"]}}