GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE341nnn/GSE341230/primaryOK200TranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE341230GEOGSEfalseDisrupting BRD4-BD1–p62 Engagement Restores Protective Autophagy [RNA-seq]Impaired protective autophagy contributes to idiopathic pulmonary fibrosis (IPF), yet therapeutic strategies that restore this process remain limited. Here, we identify SKLB-39b, a BRD4-BD1-selective inhibitor that restores protective autophagy and attenuates pulmonary fibrosis. SKLB-39b exhibits over 100-fold selectivity for BRD4-BD1 relative to BRD4-BD2 and nearly 10-fold greater affinity for BRD4-BD1 than for BRD2/3-BD1. Mechanistically, we identify a BRD4-BD1-p62 interaction that links BET bromodomain function to autophagy regulation. By engaging Leu92 and Ile146 through a hydrophobic binding mode, SKLB-39b disrupts this interaction, restores ULK1-dependent autophagy, and provides a structural framework for BD1-selective inhibitor design. SKLB-39b outperformed JQ-1 in suppressing fibroblast activation, epithelial-mesenchymal transition, and collagen deposition in experimental fibrosis while showing favorable pharmacokinetic exposure and no overt toxicity in the mouse studies performed. These findings establish the BRD4-BD1-p62 interface as a druggable target for restoring protective autophagy in IPF.2026/08/17GSE341230GSM9902472GSM9902473GSM9902474GSM9902475GSM9902470GSM9902471GSM9902476GSM9902477GSM990247824676341230Homo sapiens