GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE341nnn/GSE341320/primaryOK200Genomics Mus musculusHomo sapiensGenome binding/occupancy profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE341320GEOGSEfalseVitamin B2 Sensing by the Nuclear Receptor AhR Reprograms Hepatic Metabolism [ChIP-Seq]Nuclear receptors such as RAR/RXR and VDR directly sense vitamins A and D, but analogous sensing mechanisms for most other vitamins remain unknown. Here we identify a metabolite sensing pathway linking vitamin B2 (riboflavin) availability to an adaptive gene expression program. Riboflavin deficiency limits the FAD-dependent enzyme kynurenine 3-monooxygenase, leading to accumulation of kynurenine, an endogenous ligand of the aryl hydrocarbon receptor (AhR). Kynurenine-induced AhR nuclear translocation selectively activates poised hepatic enhancers in cooperation with AP-1 and C/EBPα, inducing glucuronidation genes and the FAD synthase gene FLAD1. This response is required to maintain intracellular FAD levels and support cell fitness under riboflavin-limiting conditions. In vivo, dietary riboflavin deficiency enhances hepatic drug clearance and reduces irinotecan toxicity. Together, these findings reveal a vitamin-sensing mechanism in which a cofactor-dependent enzyme acts as the sensor and AhR functions as the transcriptional effector that enables both flavin homeostasis and hepatic clearance capacity.2026/08/01GSE341320GSM9903837GSM9903838GSM9903839GSM9903833GSM9903834GSM9903835GSM9903836GSM9903830GSM9903831GSM9903832GSM9903848GSM9903849GSM9903844GSM9903845GSM9903846GSM9903847GSM9903840GSM9903841GSM9903842GSM9903843GSM9903850GSM9903819GSM9903815GSM9903859GSM9903816GSM9903817GSM9903818GSM9903855GSM9903856GSM9903857GSM9903858GSM9903851GSM9903852GSM9903853GSM9903854GSM9903860GSM9903861GSM9903826GSM9903827GSM9903828GSM9903829GSM9903822GSM9903866GSM9903867GSM9903823GSM9903824GSM9903868GSM9903825GSM9903862GSM9903863GSM9903864GSM9903820GSM9903821GSM9903865301733428434290341320 Mus musculusHomo sapiens