GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE341nnn/GSE341321/primaryOK200Transcriptomics Mus musculusHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE341321GEOGSEfalseVitamin B2 Sensing by the Nuclear Receptor AhR Reprograms Hepatic Metabolism [RNA-Seq]Nuclear receptors such as RAR/RXR and VDR directly sense vitamins A and D, but analogous sensing mechanisms for most other vitamins remain unknown. Here we identify a metabolite sensing pathway linking vitamin B2 (riboflavin) availability to an adaptive gene expression program. Riboflavin deficiency limits the FAD-dependent enzyme kynurenine 3-monooxygenase, leading to accumulation of kynurenine, an endogenous ligand of the aryl hydrocarbon receptor (AhR). Kynurenine-induced AhR nuclear translocation selectively activates poised hepatic enhancers in cooperation with AP-1 and C/EBPα, inducing glucuronidation genes and the FAD synthase gene FLAD1. This response is required to maintain intracellular FAD levels and support cell fitness under riboflavin-limiting conditions. In vivo, dietary riboflavin deficiency enhances hepatic drug clearance and reduces irinotecan toxicity. Together, these findings reveal a vitamin-sensing mechanism in which a cofactor-dependent enzyme acts as the sensor and AhR functions as the transcriptional effector that enables both flavin homeostasis and hepatic clearance capacity.2026/08/01GSE341321GSM9903958GSM9903959GSM9903954GSM9903955GSM9903956GSM9903957GSM9903950GSM9903951GSM9903952GSM9903953GSM9903960GSM9903965GSM9903966GSM9903967GSM9903968GSM9903961GSM9903962GSM9903963GSM9903964GSM9903907GSM9903908GSM9903909GSM9903903GSM9903904GSM9903905GSM9903906GSM9903900GSM9903901GSM9903902GSM9903869GSM9903870GSM9903871GSM9903872GSM9903918GSM9903919GSM9903914GSM9903915GSM9903916GSM9903917GSM9903877GSM9903910GSM9903911GSM9903878GSM9903912GSM9903879GSM9903913GSM9903873GSM9903874GSM9903875GSM9903876GSM9903880GSM9903881GSM9903882GSM9903883GSM9903929GSM9903925GSM9903926GSM9903927GSM9903928GSM9903888GSM9903921GSM9903889GSM9903922GSM9903923GSM9903924GSM9903884GSM9903885GSM9903886GSM9903920GSM9903887GSM9903891GSM9903892GSM9903893GSM9903894GSM9903890GSM9903936GSM9903937GSM9903938GSM9903939GSM9903932GSM9903899GSM9903933GSM9903934GSM9903935GSM9903895GSM9903896GSM9903897GSM9903930GSM9903931GSM9903898GSM9903947GSM9903948GSM9903949GSM9903943GSM9903944GSM9903945GSM9903946GSM9903940GSM9903941GSM9903942301733428434290341321 Mus musculusHomo sapiens