{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE341nnn/GSE341401/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE341401"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Investigating the potential of an α7 nicotinic acetylcholine receptor agonist in adult mammalian retinal neurogenesis through Müller glia reprogramming and progenitor cell formation","description":"Purpose: Adult mammalian Müller glia (MG) typically undergo a gliotic response after injury that inhibits neuronal regeneration. Here, we tested whether the α7 nicotinic acetylcholine receptor agonist PNU-282987 can promote MG proliferation and progenitor formation. Methods: Primary retinal pigment epithelium (RPE) cells were treated with 100 nM PNU-282987 or 0.1% DMSO, then co-cultured with primary MG cells. RNA was extracted, sequenced on the Illumina NovaSeq platform, and mapped to the Mus musculus (GRCm38) genome. Differential expression was assessed (|log2FC| ≥ 1, padj ≤ 0.05). Results: Significantly differentially expressed genes (sDEGs) were identified, including genes associated with progenitor formation, retinal development, and regeneration. Selected targets were validated by RT-qPCR. Conclusion:PNU-282987 treatment induced transcriptomic changes in MG cells consistent with progenitor formation and regenerative/developmental signaling, with more robust effects than those observed in immortalized MG lines.","dates":{"publication":"2026/08/12"},"accession":"GSE341401","cross_references":{"GSM":["GSM9906103","GSM9906102","GSM9906105","GSM9906104","GSM9906099","GSM9906098","GSM9906101","GSM9906100","GSM9906107","GSM9906106","GSM9906109","GSM9906108"],"GPL":["34328"],"GSE":["341401"],"taxon":["Mus musculus"]}}