GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE341nnn/GSE341401/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE341401GEOGSEfalseInvestigating the potential of an α7 nicotinic acetylcholine receptor agonist in adult mammalian retinal neurogenesis through Müller glia reprogramming and progenitor cell formationPurpose: Adult mammalian Müller glia (MG) typically undergo a gliotic response after injury that inhibits neuronal regeneration. Here, we tested whether the α7 nicotinic acetylcholine receptor agonist PNU-282987 can promote MG proliferation and progenitor formation. Methods: Primary retinal pigment epithelium (RPE) cells were treated with 100 nM PNU-282987 or 0.1% DMSO, then co-cultured with primary MG cells. RNA was extracted, sequenced on the Illumina NovaSeq platform, and mapped to the Mus musculus (GRCm38) genome. Differential expression was assessed (|log2FC| ≥ 1, padj ≤ 0.05). Results: Significantly differentially expressed genes (sDEGs) were identified, including genes associated with progenitor formation, retinal development, and regeneration. Selected targets were validated by RT-qPCR. Conclusion:PNU-282987 treatment induced transcriptomic changes in MG cells consistent with progenitor formation and regenerative/developmental signaling, with more robust effects than those observed in immortalized MG lines.2026/08/12GSE341401GSM9906103GSM9906102GSM9906105GSM9906104GSM9906099GSM9906098GSM9906101GSM9906100GSM9906107GSM9906106GSM9906109GSM990610834328341401Mus musculus