{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE341nnn/GSE341473/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE341473"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"RNA-seq datasets of Blood transcriptomics reveals the value of Hub genes in patients with NTM - PD","description":"To identify and validate peripheral blood mRNA signatures as non-invasive diagnostic biomarkers for nontuberculous mycobacterial pulmonary disease (NTM-PD), we performed an integrated transcriptomic analysis combining scRNA-seq and bulk RNA sequencing (RNA-seq), followed by experimental validation. Peripheral blood samples were collected from a total of 8 participants, including 3 patients with Mycobacterium avium complex pulmonary disease (MAC-PD), 3 patients with Mycobacterium abscessus pulmonary disease (MAB-PD), and 2 healthy controls (HC). All samples were subjected to scRNA-seq to dissect the single-cell transcriptomic landscape of peripheral blood, and paired bulk RNA-seq was conducted simultaneously to achieve comprehensive transcriptomic profiling. Integrated analysis of scRNA-seq and bulk RNA-seq datasets revealed that eight core genes were significantly upregulated in NTM-PD patients compared with HCs. Notably, this gene signature exhibited superior specificity relative to other candidate gene combinations, highlighting its potential as a diagnostic biomarker for NTM-PD.","dates":{"publication":"2026/08/04"},"accession":"GSE341473","cross_references":{"GSM":["GSM9907336","GSM9907337","GSM9907345","GSM9907334","GSM9907346","GSM9907335","GSM9907343","GSM9907332","GSM9907344","GSM9907333","GSM9907341","GSM9907330","GSM9907331","GSM9907342","GSM9907340","GSM9907329","GSM9907338","GSM9907327","GSM9907339","GSM9907328"],"GPL":["24676"],"GSE":["341473"],"taxon":["Homo sapiens"]}}