{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE341nnn/GSE341595/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE341595"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Alox15 restrains glycolysis to support M2 macrophage effector function during helminth immunity.","description":"Macrophage polarization by type-2 cytokines is central to anti-helminth immunity and tissue repair. While some hallmark changes in macrophages are well-characterized and associated with protection against helminths, it is still unclear how macrophages exert their anti-helminth effects. In this context, we investigated Arachidonate 15-lipoxygenase (Alox15), a lipoxygenase well known for its role in macrophage polarization in the context of metabolic diseases, and a hallmark of type-2 macrophage (M2) human polarization. We show that in the absence of Alox15, M2 cannot trap and kill helminths. Surprisingly, expression of M2 markers was normal despite a loss of function. Instead, we found a concomitant increase in pro-inflammatory responses due to an uncontrolled activation of glycolysis. We further show that activation of Peroxisome proliferator-activated receptor-delta (PPAR-δ) by lipids downstream of Docosapentaenoic acid (DPA) can restore normal glycolysis control, highlighting a novel role of lipids in the fine-tuning of the metabolic support required for optimal macrophage polarization.","dates":{"publication":"2026/08/25"},"accession":"GSE341595","cross_references":{"GSM":["GSM9913739","GSM9913717","GSM9913718","GSM9913737","GSM9913715","GSM9913716","GSM9913738","GSM9913719","GSM9913753","GSM9913731","GSM9913732","GSM9913751","GSM9913730","GSM9913752","GSM9913735","GSM9913736","GSM9913714","GSM9913733","GSM9913734","GSM9913750","GSM9913728","GSM9913729","GSM9913726","GSM9913748","GSM9913749","GSM9913727","GSM9913742","GSM9913720","GSM9913743","GSM9913721","GSM9913740","GSM9913741","GSM9913746","GSM9913724","GSM9913725","GSM9913747","GSM9913722","GSM9913744","GSM9913723","GSM9913745"],"GPL":["30215"],"GSE":["341595"],"taxon":["Mus musculus"]}}