{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE342nnn/GSE342214/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE342214"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"In vitro mechanisms of primary dermal fibroblast activation","description":"Cancer-associated fibroblasts (CAFs) are the dominant stromal cell population in melanoma but remain poorly characterized due to a lack of validated in vitro models and subtype-specific markers. To address this, primary human dermal fibroblasts were activated toward an inflammatory-like CAF (iCAF) or myofibroblast-like CAF (myCAF) phenotype using four independent induction strategies: (1) treatment with lipopolysaccharide (LPS, iCAF-like) or TGF-beta (myCAF-like); (2) CRISPR-dCas9-mediated transcriptional activation of IL6 (iCAF-like) or ACTA2 (myCAF-like); (3) direct co-culture with unstimulated or LPS-stimulated peripheral blood mononuclear cells (PBMCs); and (4) culture in conditioned medium from SK-MEL-28 melanoma cells. Activation states were validated by RT-qPCR and Western blot for the canonical markers IL6 and ACTA2 prior to RNA sequencing (Illumina NovaSeq 6000, paired-end 150 bp). Bulk RNA-seq was performed on fibroblasts from each condition and matched controls to define the transcriptome-wide gene expression changes associated with iCAF- and myCAF-like activation, enabling derivation of subtype-specific gene signatures for downstream analysis of stromal reprogramming in melanocytic malignancy.","dates":{"publication":"2026/09/01"},"accession":"GSE342214","cross_references":{"GSM":["GSM9925780","GSM9925782","GSM9925781","GSM9925784","GSM9925783","GSM9925786","GSM9925785","GSM9925788","GSM9925787","GSM9925789","GSM9925771","GSM9925770","GSM9925773","GSM9925772","GSM9925815","GSM9925775","GSM9925774","GSM9925810","GSM9925777","GSM9925776","GSM9925812","GSM9925779","GSM9925778","GSM9925811","GSM9925814","GSM9925813","GSM9925762","GSM9925761","GSM9925805","GSM9925804","GSM9925807","GSM9925806","GSM9925809","GSM9925808","GSM9925764","GSM9925763","GSM9925766","GSM9925765","GSM9925801","GSM9925768","GSM9925767","GSM9925800","GSM9925803","GSM9925769","GSM9925802","GSM9925791","GSM9925790","GSM9925793","GSM9925792","GSM9925795","GSM9925794","GSM9925797","GSM9925796","GSM9925799","GSM9925798"],"GPL":["24676"],"GSE":["342214"],"taxon":["Homo sapiens"]}}