GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE342nnn/GSE342214/primary200OKTranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE342214GEOGSEfalseIn vitro mechanisms of primary dermal fibroblast activationCancer-associated fibroblasts (CAFs) are the dominant stromal cell population in melanoma but remain poorly characterized due to a lack of validated in vitro models and subtype-specific markers. To address this, primary human dermal fibroblasts were activated toward an inflammatory-like CAF (iCAF) or myofibroblast-like CAF (myCAF) phenotype using four independent induction strategies: (1) treatment with lipopolysaccharide (LPS, iCAF-like) or TGF-beta (myCAF-like); (2) CRISPR-dCas9-mediated transcriptional activation of IL6 (iCAF-like) or ACTA2 (myCAF-like); (3) direct co-culture with unstimulated or LPS-stimulated peripheral blood mononuclear cells (PBMCs); and (4) culture in conditioned medium from SK-MEL-28 melanoma cells. Activation states were validated by RT-qPCR and Western blot for the canonical markers IL6 and ACTA2 prior to RNA sequencing (Illumina NovaSeq 6000, paired-end 150 bp). Bulk RNA-seq was performed on fibroblasts from each condition and matched controls to define the transcriptome-wide gene expression changes associated with iCAF- and myCAF-like activation, enabling derivation of subtype-specific gene signatures for downstream analysis of stromal reprogramming in melanocytic malignancy.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 sapiens