{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE342nnn/GSE342353/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"species":["Mus musculus"],"gds_type":["Genome binding/occupancy profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE342353"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"A Nanomolar-Affinity Antibody Enabling Sensitive Labeling and High-Resolution Genomic Mapping of O-GlcNAc","description":"High-affinity O-GlcNAc-specific antibodies are crucial for the sensitive detection and functional study of O‑GlcNAcylation. Here, we report the development of a pan-specific, nanomolar-affinity O-GlcNAc antibody named EPR19847 by using a degenerate O‑GlcNAcylated peptide as the immunogen. The EPR19847 antibody can be used for O‑GlcNAc detection, imaging, isolation, and chromatin immunoprecipitation. Using this novel antibody, O-GlcNAcylated proteins were enriched and identified in mouse embryonic stem cells (mESCs). Chromatin immunoprecipitation followed by next-generation sequencing (ChIP-seq) demonstrates that O-GlcNAc is enriched around the promoters of highly expressed genes, and that its genomic distribution is positively correlated with transcription-activating marks including H3K4me3, H3K9ac, and RNA Pol II in mESCs. Further analysis suggests a high occupancy of O-GlcNAcylation within super-enhancers. We anticipate that EPR19847 will serve as a powerful tool for the highly-sensitive detection and systematic functional dissection of O-GlcNAc.","dates":{"publication":"2026/08/09"},"accession":"GSE342353","cross_references":{"GSM":["GSM9929025","GSM9929024","GSM9929029","GSM9929028","GSM9929027","GSM9929026","GSM9929031","GSM9929030"],"GPL":["21273"],"GSE":["342353"],"taxon":["Mus musculus"]}}