{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE342nnn/GSE342462/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE342462"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Integrated transcriptomic and bioelectrical profiling of stem-like cellular states in a colorectal cancer using SdFFF and UHF-DEP","description":"Cancer stem-like cells (CSCs) enrichment and characterization remain an analytical challenge, as conventional marker-based approaches are often time-consuming and can alter cellular properties. Here, we develop a complete label-free protocol for the enrichment and characterization of cellular states associated to stemness properties in colorectal cancer model, based on the coupling adjustment of sedimentation field-flow fractionation (SdFFF) and ultra-high-frequency dielectrophoresis (UHF-DEP). To this end, the operating conditions of SdFFF were adapted to a low conductivity dielectrophoresis buffer while maintaining the hyperlayer elution regime. Fractionated cellular subpopulation exhibits phenotypic differences in proliferative status, clonogenic potential, stem-cell related protein levels and UHF-DEP highlights significantly different high-frequency crossover distributions. Transcriptome sequencing revealed dysregulation of pathways associated with stemness, cell adhesion, membrane organization, calcium signaling and ion-channel activity, that can be linked to dielectric signatures. Overall, UHF-DEP coupling to SdFFF permit to enrich, characterize and quantify stem cell-like subpopulation within heterogeneous population of cancer cells.","dates":{"publication":"2026/08/08"},"accession":"GSE342462","cross_references":{"GSM":["GSM9931059","GSM9931058","GSM9931055","GSM9931054","GSM9931057","GSM9931056","GSM9931051","GSM9931061","GSM9931050","GSM9931053","GSM9931052","GSM9931060"],"GPL":["28038"],"GSE":["342462"],"taxon":["Homo sapiens"]}}