{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE342nnn/GSE342686/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"species":["Mus musculus"],"gds_type":["Genome binding/occupancy profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE342686"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Transient Manganese Exposure Reprograms Bone Marrow–Derived Macrophages Toward a Stable Anti-Inflammatory Memory State [CUT&Tag]","description":"Manganese ions (Mn2+) are potent immunomodulatory agents that activate the cGAS–STING pathway. While their role as acute adjuvants is well-documented, the innate immune memory (trained immunity) signatures induced by Mn2+ remain unexplored. Using an in vitro priming–rest–challenge model, we demonstrate that transient Mn2+ stimulation induces a persistent anti-inflammatory state in bone marrow–derived macrophages (BMDM). Unlike the immediate pro-inflammatory burst associated with Mn2+, Mn2+-trained BMDMs displayed a stable reduction in Il6, Il1b, Il12a, and Nos2 expression upon secondary LPS or LPS+IFNγ challenge. Furthermore, Mn2+ training enhanced IL-4-driven Arg1 induction and suppressed MHC II surface expression, antigen presentation pathways, and phagocytic capacity against Candida albicans. Mechanistically, this functional attenuation was independent of glycolysis but correlated with a broad downregulation of lipid metabolic pathways. These findings reveal a lineage-specific regulatory \"brake\" induced by manganese, providing critical insights for its application in immunotherapy.","dates":{"publication":"2026/09/04"},"accession":"GSE342686","cross_references":{"GSM":["GSM9936450","GSM9936451","GSM9936452","GSM9936453","GSM9936454","GSM9936449"],"GPL":["24247"],"GSE":["342686"],"taxon":["Mus musculus"]}}