{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE342nnn/GSE342896/"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"omics_type":["Genomics"],"species":["Mus musculus"],"gds_type":["Genome binding/occupancy profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE342896"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Sharp cell type boundaries emerge from coordinated morphogen signaling [CUT&Run]","description":"Classic models of the French flag problem depict sharp cell-type boundaries emerging from threshold responses to morphogen gradients. How discrete cell-type boundaries arise from morphogen signals that vary continuously across developing tissues remains incompletely understood. We use hair follicle dermal condensate (DC) formation to study a sharp developmental transition in which proliferative progenitors undergo cell-cycle exit concurrent with molecular differentiation. Using genetic and genomic approaches, we show that Wnt and Hedgehog signaling coordinate separable cellular events during DC commitment. Elevated Wnt signaling promotes cell-cycle exit by reducing chromatin binding of the Hedgehog mediator GLI3, while Hedgehog signaling induces differentiation genes in a Wnt-dependent manner and simultaneously elevates Wnt activity. When these responses coincide, differentiation and cell-cycle exit occur together, limiting the duration and abundance of intermediate states and producing a sharp boundary. When they do not, intermediate states persist and expand, producing a graded boundary. Thus, a sharp boundary can emerge from a continuous transition that is compressed in time and space.","dates":{"publication":"2026/08/25"},"accession":"GSE342896","cross_references":{"GSM":["GSM9941871","GSM9941872","GSM9941850","GSM9941851","GSM9941873","GSM9941874","GSM9941852","GSM9941870","GSM9941857","GSM9941879","GSM9941858","GSM9941859","GSM9941875","GSM9941853","GSM9941854","GSM9941876","GSM9941877","GSM9941855","GSM9941878","GSM9941856","GSM9941860","GSM9941882","GSM9941883","GSM9941861","GSM9941884","GSM9941862","GSM9941863","GSM9941880","GSM9941881","GSM9941868","GSM9941846","GSM9941869","GSM9941847","GSM9941848","GSM9941849","GSM9941842","GSM9941864","GSM9941865","GSM9941843","GSM9941866","GSM9941844","GSM9941845","GSM9941867"],"GPL":["24247"],"GSE":["342896"],"taxon":["Mus musculus"]}}