GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE342nnn/GSE342977/primaryOK200TranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE342977GEOGSEfalseTranscriptional consequences of viral and cellular ORF overexpression in hTERT-RPE1 cellsTo define the transcriptional programs driven by individual viral and cellular open reading frames, hTERT-RPE1 cells were stably transduced with lentiviral constructs expressing GFP11 (negative control), the human papillomavirus E7 oncoprotein, the Epstein-Barr virus protein BNLF2b, or the cAMP-dependent protein kinase inhibitor alpha (PKIA). After puromycin selection, total RNA was profiled by 3' end counting RNA-seq in four biological replicates per condition. Comparison of each ORF-expressing population against the GFP11 control defines the gene expression changes attributable to that ORF.2026/08/13GSE342977GSM9944295GSM9944294GSM9944297GSM9944296GSM9944291GSM9944290GSM9944293GSM9944292GSM9944302GSM9944299GSM9944288GSM9944287GSM9944298GSM9944301GSM9944300GSM994428934284342977Homo sapiens