{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE343nnn/GSE343368/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"species":["Homo sapiens"],"gds_type":["Genome binding/occupancy profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE343368"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"NWU Study 2- ATAC-seq timecourse of KOLF2.2J hiPSC after Auxin-induced protein degradation of specific gene products","description":"This study investigates the impact of gene products on chromatin accessibility in growing hiPSC at 0, 6 & 24 hours after acute protein depletion. Crispr editing was used to add an auxin-dependent degron to the C-termini of gene products expressed from the endogeneous loci and to insert an auxin-responsive degradation E3 ligase to the AAVS locus. Rapid (<30 min), specific target protein degradation in KOLF2.2J hiPSCs was induced by a synthetic auxin derivative (5-Ad-IAA) and bulk ATAC-seq was performed at timepoints to define early (6 hr) and later (24 hr) downstream chromatin remodeling.","dates":{"publication":"2026/08/12"},"accession":"GSE343368","cross_references":{"GSM":["GSM9949797","GSM9949830","GSM9949796","GSM9949795","GSM9949794","GSM9949793","GSM9949792","GSM9949791","GSM9949790","GSM9949838","GSM9949837","GSM9949836","GSM9949835","GSM9949834","GSM9949833","GSM9949832","GSM9949799","GSM9949831","GSM9949798","GSM9949839","GSM9949841","GSM9949840","GSM9949849","GSM9949848","GSM9949847","GSM9949846","GSM9949845","GSM9949844","GSM9949843","GSM9949842","GSM9949896","GSM9949895","GSM9949894","GSM9949893","GSM9949892","GSM9949891","GSM9949890","GSM9949816","GSM9949815","GSM9949814","GSM9949813","GSM9949812","GSM9949811","GSM9949899","GSM9949898","GSM9949810","GSM9949897","GSM9949819","GSM9949818","GSM9949817","GSM9949827","GSM9949826","GSM9949825","GSM9949824","GSM9949823","GSM9949822","GSM9949789","GSM9949788","GSM9949821","GSM9949820","GSM9949829","GSM9949828","GSM9949874","GSM9949873","GSM9949872","GSM9949871","GSM9949870","GSM9949913","GSM9949912","GSM9949879","GSM9949911","GSM9949878","GSM9949877","GSM9949910","GSM9949876","GSM9949875","GSM9949885","GSM9949884","GSM9949883","GSM9949882","GSM9949881","GSM9949880","GSM9949805","GSM9949804","GSM9949803","GSM9949802","GSM9949889","GSM9949801","GSM9949888","GSM9949800","GSM9949887","GSM9949886","GSM9949809","GSM9949808","GSM9949807","GSM9949806","GSM9949852","GSM9949851","GSM9949850","GSM9949859","GSM9949858","GSM9949857","GSM9949856","GSM9949855","GSM9949854","GSM9949853","GSM9949863","GSM9949862","GSM9949861","GSM9949860","GSM9949904","GSM9949903","GSM9949869","GSM9949902","GSM9949868","GSM9949901","GSM9949900","GSM9949867","GSM9949866","GSM9949865","GSM9949864","GSM9949909","GSM9949908","GSM9949907","GSM9949906","GSM9949905"],"GPL":["24676"],"GSE":["343368"],"taxon":["Homo sapiens"]}}