{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE343nnn/GSE343560/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE343560"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"RNA sequencing of mouse palatal epithelial cells following TGF-β3 treatment or Tgfb3 knockdown","description":"Epithelial-mesenchymal transition (EMT) of medial edge epithelial cells is a critical mechanism underlying embryonic palatal fusion, driven by transforming growth factor beta 3 (TGF-β3). However, its regulatory interplay with downstream cascades remains poorly understood. To elucidate cellular reprogramming mechanisms during palatogenesis, embryonic day 15 C57BL6/J mouse palatal epithelial cells were isolated to eliminate mesenchymal interference. To evaluate transcriptomic shifts, next-generation RNA sequencing was performed on cells cultured in three groups: a control group (no treatment), a group treated with 1 ng/mL of recombinant TGF-β3 to induce EMT, and a group with siRNA transfection Tgfb3 knockdown. This dataset provides the global gene expression profiles underlying TGF-β3-mediated palatal fusion, revealing EMT as the main pathway.","dates":{"publication":"2026/09/11"},"accession":"GSE343560","cross_references":{"GSM":["GSM9957257","GSM9957258","GSM9957259","GSM9957260","GSM9957261"],"GPL":["24247"],"GSE":["343560"],"taxon":["Mus musculus"]}}