{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE343nnn/GSE343956/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE343956"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Transcriptional co-regulator Med15 is required to maintain functional maturation in adult β-cells","description":"Transcriptional coregulators, such as the Mediator complex, partner with transcription factors to regulate gene transcription throughout all stages of life. Subunit Med15 is required for pre-natal development and post-natal maturation of pancreatic β-cells. However, whether Med15 continues to play a role in β-cell function after maturation is complete, and throughout adulthood, is unknown. To investigate the role of Med15 in β-cells post-maturation, we induced a β-cell specific Med15 knockout at six weeks age in male and female mice. Ablation of Med15 led to glucose intolerance and impaired insulin secretion. RNA-sequencing revealed downregulation of β-cell maturation markers. Further, we implanted insulin pellets into knockout mice to lower blood glucose and performed RNA-seq to validate that the transcriptional changes were a direct consequence of the Med15 loss and not due to hyperglycemia in the knockout mice. This study shows that Med15 is required after weaning and throughout life to maintain functional β-cell maturity.","dates":{"publication":"2026/08/19"},"accession":"GSE343956","cross_references":{"GSM":["GSM9965560","GSM9965563","GSM9965564","GSM9965561","GSM9965562","GSM9965545","GSM9965567","GSM9965568","GSM9965546","GSM9965565","GSM9965566","GSM9965549","GSM9965569","GSM9965547","GSM9965548","GSM9965570","GSM9965571","GSM9965552","GSM9965553","GSM9965572","GSM9965550","GSM9965551","GSM9965556","GSM9965557","GSM9965554","GSM9965555","GSM9965558","GSM9965559"],"GPL":["30172"],"GSE":["343956"],"taxon":["Mus musculus"]}}