GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE343nnn/GSE343956/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE343956GEOGSEfalseTranscriptional co-regulator Med15 is required to maintain functional maturation in adult β-cellsTranscriptional coregulators, such as the Mediator complex, partner with transcription factors to regulate gene transcription throughout all stages of life. Subunit Med15 is required for pre-natal development and post-natal maturation of pancreatic β-cells. However, whether Med15 continues to play a role in β-cell function after maturation is complete, and throughout adulthood, is unknown. To investigate the role of Med15 in β-cells post-maturation, we induced a β-cell specific Med15 knockout at six weeks age in male and female mice. Ablation of Med15 led to glucose intolerance and impaired insulin secretion. RNA-sequencing revealed downregulation of β-cell maturation markers. Further, we implanted insulin pellets into knockout mice to lower blood glucose and performed RNA-seq to validate that the transcriptional changes were a direct consequence of the Med15 loss and not due to hyperglycemia in the knockout mice. This study shows that Med15 is required after weaning and throughout life to maintain functional β-cell maturity.2026/08/19GSE343956GSM9965560GSM9965563GSM9965564GSM9965561GSM9965562GSM9965545GSM9965567GSM9965568GSM9965546GSM9965565GSM9965566GSM9965549GSM9965569GSM9965547GSM9965548GSM9965570GSM9965571GSM9965552GSM9965553GSM9965572GSM9965550GSM9965551GSM9965556GSM9965557GSM9965554GSM9965555GSM9965558GSM996555930172343956Mus musculus