{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE343nnn/GSE343958/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE343958"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Bulk RNA-seq of HCT116 colorectal cancer 3D spheroids treated with 5-fluorouracil or olaparib","description":"This study accompanies an adapted Cell Painting assay applied to 3D colorectal cancer spheroids (BioStudies S-BIAD2254). To relate image-based morphological profiles to transcriptional responses, bulk 3'-end RNA-seq was performed on HCT116 spheroids grown in ultra-low attachment 384-well plates and treated for 48 h with 5-fluorouracil (10 uM), olaparib (3 uM), or 0.1% DMSO vehicle. Gene-level differential expression and Hallmark pathway enrichment were used to characterise the drug-induced transcriptional programs underlying the observed morphological phenotypes.","dates":{"publication":"2026/08/18"},"accession":"GSE343958","cross_references":{"GSM":["GSM9965673","GSM9965674","GSM9965682","GSM9965671","GSM9965672","GSM9965677","GSM9965678","GSM9965675","GSM9965676","GSM9965679","GSM9965680","GSM9965681"],"GPL":["34284"],"GSE":["343958"],"taxon":["Homo sapiens"]}}