GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE343nnn/GSE343958/primaryOK200TranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE343958GEOGSEfalseBulk RNA-seq of HCT116 colorectal cancer 3D spheroids treated with 5-fluorouracil or olaparibThis study accompanies an adapted Cell Painting assay applied to 3D colorectal cancer spheroids (BioStudies S-BIAD2254). To relate image-based morphological profiles to transcriptional responses, bulk 3'-end RNA-seq was performed on HCT116 spheroids grown in ultra-low attachment 384-well plates and treated for 48 h with 5-fluorouracil (10 uM), olaparib (3 uM), or 0.1% DMSO vehicle. Gene-level differential expression and Hallmark pathway enrichment were used to characterise the drug-induced transcriptional programs underlying the observed morphological phenotypes.2026/08/18GSE343958GSM9965673GSM9965674GSM9965682GSM9965671GSM9965672GSM9965677GSM9965678GSM9965675GSM9965676GSM9965679GSM9965680GSM996568134284343958Homo sapiens