GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE344nnn/GSE344004/primaryOK200TranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE344004GEOGSEfalseTranscriptomic profiling of NCI-H441 epithelial cells following BCG exposure in a human cell-line lung-on-chip modelThis study investigated early transcriptional responses of NCI-H441 lung epithelial cells in a human cell-line lung-on-chip (CLLOC) model following epithelial-side exposure to Bacillus Calmette-Guérin (BCG). The CLLOC model contained NCI-H441 epithelial cells in the epithelial compartment and human umbilical vein endothelial cells (HUVECs) in the vascular compartment, with human monocyte-derived macrophages incorporated into the epithelial side. BCG was applied to the epithelial/apical compartment, while phosphate-buffered saline (PBS) was used as the control. NCI-H441 epithelial cells were collected from CLLOCs at 12 h post-stimulation for bulk RNA sequencing.2026/09/30GSE344004GSM9966166GSM9966167GSM9966164GSM9966165GSM9966162GSM996616334284344004Homo sapiens