{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE344nnn/GSE344050/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE344050"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Assessment of the association of periodontitis and diabetes mellitus with Alzheimer's disease in a mouse model [RNA-seq]","description":"The purpose of this study was to investigate the association of periodontitis and diabetes mellitus (DM) with Alzheimer’s disease (AD)-related changes through microRNA (miRNA) using AD model mice. Twenty-four male knock-in mice (B6-AppNL-G-F/NL-G-F/J) were divided into four groups: control (C), DM, periodontitis (P), and DM plus periodontitis (DM+P). After an 8-week experimental period, memory performance was assessed using the Y-maze test, and brain samples were analyzed by next-generation sequencing. miRNA and mRNA expression profiles were evaluated by comparing the DM+P and C groups, and integrated miRNA–mRNA analysis was performed to identify candidate regulatory relationships. Neurod1 protein expression in the hippocampus was also assessed. The DM+P group showed significantly lower memory performance than the C group. Integrated analysis identified seven candidate miRNA–mRNA pairs, among which Neurod1 showed the greatest decrease in mRNA expression and was predicted to be regulated by miR-693-3p. Neurod1 protein expression in the hippocampus was significantly lower in the DM+P group than in the C group. These findings suggest that combined exposure to periodontitis and DM was associated with AD-like pathological changes and alterations in miRNA–mRNA regulatory relationships, with the miR-693-3p/Neurod1 pair identified as a candidate regulatory axis.","dates":{"publication":"2026/08/22"},"accession":"GSE344050","cross_references":{"GSM":["GSM9967068","GSM9967066","GSM9967067","GSM9967065"],"GPL":["37305"],"GSE":["344050"],"taxon":["Mus musculus"]}}