GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE344nnn/GSE344097/primaryOK200TranscriptomicsRattus norvegicusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE344097GEOGSEfalseNobiletin targets USP5 to inhibit ferroptosis and alleviate doxorubicin-induced cardiotoxicityDoxorubicin (DOX) is an effective chemotherapeutic agent that can cause cardiotoxicity and myocardial injury. Nobiletin (NOB), a natural polymethoxyflavone, has been reported to exert protective effects in cardiovascular disease models. To investigate the transcriptional changes associated with DOX-induced cellular injury and the potential protective effects of NOB, RNA sequencing was performed in H9c2 rat cardiomyoblasts subjected to different treatments. H9c2 cells were divided into four groups: control (CON), nobiletin-treated (NOB), doxorubicin-treated (DOX), and doxorubicin plus nobiletin-treated (DOX+NOB) groups, with three biological replicates per group. Cells were treated with 100 μM NOB and/or 1 μM DOX for 24 h, according to the experimental group. Transcriptomic profiles were generated using paired-end sequencing on an Illumina NovaSeq 6000 platform. The resulting RNA-seq dataset provides a resource for characterizing transcriptional responses to DOX exposure and investigating the potential molecular effects of NOB in the context of DOX-induced cellular injury.2026/08/23GSE344097GSM9970589GSM9970600GSM9970597GSM9970596GSM9970599GSM9970598GSM9970593GSM9970592GSM9970595GSM9970594GSM9970591GSM997059025947344097Rattus norvegicus