GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE344nnn/GSE344100/primaryOK200TranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE344100GEOGSEfalseEffects of 3D and 2D culture on gene expression of HCT116 cellsAccurate preclinical models that recapitulate in vivo tumor pathophysiology are essential for reliable assessment of anticancer drug efficacy. Three-dimensional (3D) cell culture systems, which better mimic the in vivo microenvironment, have garnered significant attention. However, high throughput production of uniformly sized spheroids remains a key challenge for drug screening. we developed a PDMS substrate with hexagonally arrayed U-shaped microwells (HAUM PDMS) for high-throughput culture of uniformly sized spheroids. Transcriptome sequencing was performed on HCT116 spheroids harvested from 500 µm HAUM PDMS substrates and on 2D-cultured cells after 3 days. The results showed that transcriptome analysis indicated that up-regulated genes in spheroids were mainly related to cell division and proliferation.2026/08/18GSE344100GSM9970620GSM9970619GSM9970616GSM9970615GSM9970618GSM997061724676344100Homo sapiens