GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE344nnn/GSE344216/primaryOK200TranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE344216GEOGSEfalseSingle-nucleus transcriptomics reveals coordinated neuronal, immune, and glial dysregulation in the dorsolateral prefrontal cortex in major depressive disorderWe used single-nucleus RNA sequencing to define cell-type-specific molecular alterations in the dorsolateral prefrontal cortex (dlPFC) of individuals with MDD. We assessed cell-type composition, differential expression, pathway enrichment, oligodendrocyte-lineage pseudotime, ligand-receptor communication, transcription-factor regulon activity, and overlap with MDD-linked genetic variants. The integrated atlas resolved excitatory and inhibitory neuronal subclasses, oligodendrocyte-lineage cells, astrocytes, microglia/perivascular macrophages, endothelial cells, and vascular-associated populations.2026/08/24GSE344216GSM9973084GSM9973083GSM9973082GSM9973081GSM9973080GSM9973109GSM9973108GSM9973107GSM9973106GSM9973105GSM9973104GSM9973103GSM9973069GSM9973102GSM9973101GSM9973100GSM9973089GSM9973088GSM9973087GSM9973086GSM9973085GSM9973095GSM9973073GSM9973072GSM9973094GSM9973093GSM9973071GSM9973092GSM9973070GSM9973091GSM9973090GSM9973114GSM9973113GSM9973112GSM9973079GSM9973078GSM9973111GSM9973077GSM9973110GSM9973099GSM9973098GSM9973076GSM9973075GSM9973097GSM9973074GSM997309624676344216Homo sapiens