{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE344nnn/GSE344232/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE344232"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"HOXA3 regulates thymic epithelial cell differentiation from hESCs and supports T-cell differentiation through CXCL12","description":"H9 human embryonic stem cells were differentiated into thymic epithelial cells (TECs) via a three-stage protocol. HOXA3 was knocked down during the 3PPE-to-TEC transition to investigate its role in TEC differentiation and function. RNA-seq was performed on HOXA3 knockdown and control TEC-like cells (3 biological replicates each). HOXA3 knockdown disrupted epithelial differentiation and reduced TEC-associated gene expression, including K8, AIRE, and CD205, along with decreased FOXN1 protein. HOXA3-deficient TECs showed impaired support for T-cell generation from CD34+ progenitors. CXCL12 was identified as a downregulated gene and confirmed at mRNA and protein levels. Exogenous CXCL12 partially rescued the impaired T-cell-supporting function. This dataset provides insights into molecular regulation of human TEC differentiation.","dates":{"publication":"2026/08/24"},"accession":"GSE344232","cross_references":{"GSM":["GSM9973282","GSM9973281","GSM9973280","GSM9973279","GSM9973284","GSM9973283"],"GPL":["24676"],"GSE":["344232"],"taxon":["Homo sapiens"]}}