GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE344nnn/GSE344388/primaryOK200TranscriptomicsRattus norvegicusExpression profiling by arrayhttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE344388GEOGSEfalseIdentification of osteopontin as a positional and functional candidate gene for cardiac hypertrophy in the SHRSP ratLeft ventricular mass is a highly heritable, polygenic trait and linkage studies in WKY and SHRSP rats identified a quantitative trait locus for left ventricular mass index on chromosome 14. Congenic strains, where trait-associated genetic loci are introduced into “healthy backgrounds, identify causal mediators relevant to human disease. Chromosome 14 congenic (WKY.SPGla14a), WKY, and SHRSP strains underwent cardiac phenotyping and transcriptome profiling at; 1-3 days (neonate), 5 weeks, and 16-weeks). Gene expression was assessed in the whole heart of 1-day neonate rats, and the LV of 5 and 16 week rats (N=3/strain) using the RatRef-12 v1.0 microarray chip (Illumina). Whole hearts from 1-day neonate animals were collected, weighted and snap frozen for RNA extraction. LV was snap frozen following dissection and weighting from 5 and 16 week rats. RNA was extracted from frozen heart/LV tissue using RNeasy kits according to the manufacturer’s protocol (Qiagen). Biotinylated amplified target cDNA was prepared and hybridised to the chip.2026/08/27GSE344388GSM9978234GSM9978233GSM9978214GSM9978236GSM9978235GSM9978238GSM9978216GSM9978237GSM9978215GSM9978218GSM9978217GSM9978239GSM9978219GSM9978230GSM9978232GSM9978231GSM9978245GSM9978223GSM9978244GSM9978222GSM9978225GSM9978247GSM9978224GSM9978246GSM9978227GSM9978249GSM9978248GSM9978226GSM9978229GSM9978228GSM9978241GSM9978240GSM9978243GSM9978221GSM9978242GSM99782206101344388Rattus norvegicus