{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE344nnn/GSE344759/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Sus scrofa"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE344759"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Cryoprotectant formulation differentially shapes cardiac and immune transcriptomes in frozen storage","description":"Cardiac tissue slices are used widely for biobanking and pharmacological testing, yet how freezing and cryoprotection reshape their molecular state remains poorly defined. We stored porcine cardiac tissue slices at -20 °C in a conventional DMSO-containing cryomedium or DMSO-free trehalose-based formulations, with or without supplementation, and profiled transcriptomes from day 1 to day 7 together with TUNEL apoptosis and CD68 immunophenotyping. Transcriptional injury was formulation- and time-dependent, caused by freezing rather than 4 °C handling. The DMSO-containing cryomedium drove collapse of sarcomeric, calcium-handling and mitochondrial programmes, with key transcripts falling by -3 to -4.4 log2FC at day 7. DMSO-free storage preserved these programmes substantially better. The four supplemental protectants recovered myocardial metabolic programmes and attenuated necroptotic and ferroptotic signalling. Tissue-resident immunity was the most striking finding: complement, phagocytic and NET-related transcripts were preserved at day 1 in DMSO-containing storage, fell sharply in DMSO-free storage, and partially recovered by day 7, whereas DMSO-stored tissue showed progressive immune-associated activation. Immunophenotyping revealed formulation-dependent CD68+ cell density that mirrored the transcriptomic immune signature.","dates":{"publication":"2026/09/01"},"accession":"GSE344759","cross_references":{"GSM":["GSM9984758","GSM9984779","GSM9984757","GSM9984759","GSM9984754","GSM9984776","GSM9984775","GSM9984753","GSM9984778","GSM9984756","GSM9984755","GSM9984777","GSM9984794","GSM9984772","GSM9984793","GSM9984771","GSM9984796","GSM9984774","GSM9984773","GSM9984795","GSM9984790","GSM9984770","GSM9984792","GSM9984791","GSM9984769","GSM9984768","GSM9984787","GSM9984765","GSM9984764","GSM9984786","GSM9984767","GSM9984789","GSM9984788","GSM9984766","GSM9984761","GSM9984783","GSM9984782","GSM9984760","GSM9984785","GSM9984763","GSM9984784","GSM9984762","GSM9984781","GSM9984780"],"GPL":["30998"],"GSE":["344759"],"taxon":["Sus scrofa"]}}