GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE344nnn/GSE344759/primaryOK200TranscriptomicsSus scrofaExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE344759GEOGSEfalseCryoprotectant formulation differentially shapes cardiac and immune transcriptomes in frozen storageCardiac tissue slices are used widely for biobanking and pharmacological testing, yet how freezing and cryoprotection reshape their molecular state remains poorly defined. We stored porcine cardiac tissue slices at -20 °C in a conventional DMSO-containing cryomedium or DMSO-free trehalose-based formulations, with or without supplementation, and profiled transcriptomes from day 1 to day 7 together with TUNEL apoptosis and CD68 immunophenotyping. Transcriptional injury was formulation- and time-dependent, caused by freezing rather than 4 °C handling. The DMSO-containing cryomedium drove collapse of sarcomeric, calcium-handling and mitochondrial programmes, with key transcripts falling by -3 to -4.4 log2FC at day 7. DMSO-free storage preserved these programmes substantially better. The four supplemental protectants recovered myocardial metabolic programmes and attenuated necroptotic and ferroptotic signalling. Tissue-resident immunity was the most striking finding: complement, phagocytic and NET-related transcripts were preserved at day 1 in DMSO-containing storage, fell sharply in DMSO-free storage, and partially recovered by day 7, whereas DMSO-stored tissue showed progressive immune-associated activation. Immunophenotyping revealed formulation-dependent CD68+ cell density that mirrored the transcriptomic immune signature.2026/09/01GSE344759GSM9984758GSM9984779GSM9984757GSM9984759GSM9984754GSM9984776GSM9984775GSM9984753GSM9984778GSM9984756GSM9984755GSM9984777GSM9984794GSM9984772GSM9984793GSM9984771GSM9984796GSM9984774GSM9984773GSM9984795GSM9984790GSM9984770GSM9984792GSM9984791GSM9984769GSM9984768GSM9984787GSM9984765GSM9984764GSM9984786GSM9984767GSM9984789GSM9984788GSM9984766GSM9984761GSM9984783GSM9984782GSM9984760GSM9984785GSM9984763GSM9984784GSM9984762GSM9984781GSM998478030998344759Sus scrofa