{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE344nnn/GSE344787/"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Rattus norvegicus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE344787"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Decoding Immune Cell Subset Crosstalk in Biomaterial-Mediated Bone Regeneration: A Sequential Two-Stage Neutrophil-Macrophage Circuit","description":"This study uses single-cell RNA sequencing to profile the early immune cell landscape during biomaterial-mediated bone regeneration in a rat calvarial defect model treated with a Janus microsphere delivery system. We identify a sequential two-stage immune circuit wherein CXCL2+ macrophages recruit pro-inflammatory N1 neutrophils via CXCL2-CXCR2 to initiate inflammation, while Neu2 neutrophils subsequently drive macrophage M2 polarization through Lgals9-CD44 to resolve inflammation and initiate osteogenesis-angiogenesis coupling. These findings reveal novel immune subset crosstalk mechanisms and provide a theoretical basis for designing immunomodulatory bone repair materials.","dates":{"publication":"2026/08/24"},"accession":"GSE344787","cross_references":{"GSM":["GSM9985118","GSM9985116","GSM9985117","GSM9985114","GSM9985115","GSM9985113"],"GPL":["34316"],"GSE":["344787"],"taxon":["Rattus norvegicus"]}}