GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE344nnn/GSE344787/primary200OKTranscriptomicsRattus norvegicusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE344787GEOGSEfalseDecoding Immune Cell Subset Crosstalk in Biomaterial-Mediated Bone Regeneration: A Sequential Two-Stage Neutrophil-Macrophage CircuitThis study uses single-cell RNA sequencing to profile the early immune cell landscape during biomaterial-mediated bone regeneration in a rat calvarial defect model treated with a Janus microsphere delivery system. We identify a sequential two-stage immune circuit wherein CXCL2+ macrophages recruit pro-inflammatory N1 neutrophils via CXCL2-CXCR2 to initiate inflammation, while Neu2 neutrophils subsequently drive macrophage M2 polarization through Lgals9-CD44 to resolve inflammation and initiate osteogenesis-angiogenesis coupling. These findings reveal novel immune subset crosstalk mechanisms and provide a theoretical basis for designing immunomodulatory bone repair materials.2026/08/24GSE344787GSM9985118GSM9985116GSM9985117GSM9985114GSM9985115GSM998511334316344787Rattus norvegicus