{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE344nnn/GSE344912/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Arabidopsis thaliana"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE344912"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"GLABRA2 regulates gene expression via its own EAR-motif mediated recruitment of the TPL/TPR corepressors","description":"The Arabidopsis HD-Zip IV transcription factor GLABRA2 (GL2) displays dual regulatory capabilities as an activator and repressor of genes that mediate cell-type differentiation of the epidermis. GL2 binds L1 box elements in the promoters of its target genes; however, the mechanisms by which it controls gene expression remain elusive. GL2 contains two putative ethylene-responsive element-binding factor-associated amphiphilic repression (EAR) motifs proximal to its N- and C-termini. The N-terminal EAR motif is highly conserved among GL2 orthologs that form a distinct clade of HD-Zip IV transcription factors in monocots and dicots. We demonstrate that deletion or alanine substitution of this N-terminal EAR motif results in a partial loss-of-function phenotypes in trichomes, non-hair root cells, and seed coat mucilage. In contrast, mutations affecting the C-terminal EAR motif display improper nuclear localization, likely due to protein misfolding. Yeast two-hybrid and in planta co-immunoprecipitation assays show that GL2 selectively interacts with the TOPLESS (TPL) and TPL-RELATED (TPR) corepressors via its N-terminal EAR motif. Fusion of the SUPERMAN REPRESSIVE DOMAIN X (SRDX) with the gl2 N-terminal EAR motif mutant (gl2EAR-N) rescues the trichome and root epidermal developmental phenotypes of gl2 mutants. RNA sequencing of mutant and wild-type seedling roots further indicates the role of the GL2 N-terminal EAR motif in regulating gene expression. Our findings support a model whereby GL2 recruits TPL/TPR corepressors via its EAR motif to sequester histone-modifying proteins, resulting in chromatin remodeling required for epidermal development.","dates":{"publication":"2026/08/25"},"accession":"GSE344912","cross_references":{"GSM":["GSM9987890","GSM9987889","GSM9987888","GSM9987896","GSM9987895","GSM9987894","GSM9987893","GSM9987892","GSM9987891"],"GPL":["26208"],"GSE":["344912"],"taxon":["Arabidopsis thaliana"]}}