{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE344nnn/GSE344976/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE344976"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Gut Bacterial Tryptophanase Activity Suprresses Ethanol-Induced Liver Injury","description":"Alcohol consumption leads to disruptions to the gut microbiota including a depletion of tryptophan-derived indole metabolites. Disrupted tryptophan metabolism is clearly associated with alcohol-associated liver disease, yet our understanding of the specific role of gut microbe-driven tryptophan metabolism has been hampered due to lack of mouse models. To address this, we created a defined synthetic microbial community with either intact or genetically deleted tryptophanase activity for in vivo studies. Gnotobiotic mice lacking bacterial tryptophanase activity are more susceptible to ethanol-induced liver injury. Interestingly, mice with intact microbial tryptophanase activity have a robust induction of IL-6 and acute phase proteins when challenged with ethanol. Furthermore, bacterial tryptophanase activity promoted a spatial reorganization of the gut microbiome and colonization of Akkermansia muciniphila within the ileum. Collectively, this study highlights the functional importance of bacterial tryptophan metabolites in shaping ethanol-driven reorganization of the gut microbiome and host immune response to promote liver injury.","dates":{"publication":"2026/08/25"},"accession":"GSE344976","cross_references":{"GSM":["GSM9989092","GSM9989093","GSM9989094","GSM9989095","GSM9989096","GSM9989097","GSM9989098","GSM9989099","GSM9989110","GSM9989111","GSM9989100","GSM9989112","GSM9989101","GSM9989113","GSM9989102","GSM9989114","GSM9989103","GSM9989104","GSM9989105","GSM9989106","GSM9989107","GSM9989108","GSM9989109"],"GPL":["24247"],"GSE":["344976"],"taxon":["Mus musculus"]}}