GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE344nnn/GSE344976/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE344976GEOGSEfalseGut Bacterial Tryptophanase Activity Suprresses Ethanol-Induced Liver InjuryAlcohol consumption leads to disruptions to the gut microbiota including a depletion of tryptophan-derived indole metabolites. Disrupted tryptophan metabolism is clearly associated with alcohol-associated liver disease, yet our understanding of the specific role of gut microbe-driven tryptophan metabolism has been hampered due to lack of mouse models. To address this, we created a defined synthetic microbial community with either intact or genetically deleted tryptophanase activity for in vivo studies. Gnotobiotic mice lacking bacterial tryptophanase activity are more susceptible to ethanol-induced liver injury. Interestingly, mice with intact microbial tryptophanase activity have a robust induction of IL-6 and acute phase proteins when challenged with ethanol. Furthermore, bacterial tryptophanase activity promoted a spatial reorganization of the gut microbiome and colonization of Akkermansia muciniphila within the ileum. Collectively, this study highlights the functional importance of bacterial tryptophan metabolites in shaping ethanol-driven reorganization of the gut microbiome and host immune response to promote liver injury.2026/08/25GSE344976GSM9989092GSM9989093GSM9989094GSM9989095GSM9989096GSM9989097GSM9989098GSM9989099GSM9989110GSM9989111GSM9989100GSM9989112GSM9989101GSM9989113GSM9989102GSM9989114GSM9989103GSM9989104GSM9989105GSM9989106GSM9989107GSM9989108GSM998910924247344976Mus musculus